Palavras-chave
Flora microbiana, implantes dentales, cámara interna, control de la infección.
Como Citar
Estudio de medidas de control de la colonización microbiana de la cámara interna del implante y del sistema implante-pilar por medio de un estudio in vitro. (2019). Odontoestomatología, 19(especial), 68-76. https://odon.edu.uy/ojs/index.php/ode/article/view/182
Resumo
Objetivo: Determinar mediante un ensayo in vitro, medidas de control del bioflm en la cámara interna del implante. Método: Se seleccionaron diferentes agentes antimicrobianos que se colocaron en las cámaras de 3 grupos de implantes. Luego de 7 días de inmersos en una suspensión microbiana e
incubados, realizamos la toma, cultivo e incubación de las muestras de las cámaras de cada grupo de estudio. Resultados: Se constató una fltración de
microorganismos hacia la cámara interna en todos los grupos de implantes estudiados, obteniéndose un mayor recuento de Unidades Formadoras de Colonias (UFC) en el grupo control, mientras que en los grupos experimentales se identifcó una reducción signifcativa en el recuento de UFC.
Conclusiones: Se observó una disminución signifcativa en la cantidad de UFC en los grupos experimentales respecto al grupo control, lo que determina la ventaja de utilizar este tipo de antimicrobianos
Referências
1. Gotfredsen K, Berglundh T, Lindhe J. Anchorage of titanium implant with different surface characteristics: an experimental study in rabbits. Clin Implant Dent Relat. Res.2000; 2(3): 120-128
2. Quirynen M, van Steenberghe D. Bacterial colonization of the internal part of two-stage implants. An in vivo study. Clin Oral Implant Res. 1993; 4: 158-161.
3. Broggini N, McManus LM, Herman JS, Medina RU, Oates TW, Schenk RK, Buser D, Mellonig JT, Cochran DL. Persistent Acute Inflammation at the Implant-Abutment Interface. J Dent Res. 2003; 82(3): 232-237.
4. Besimo C, Guindy J, Lewetag D, Meyer J. Prevention of bacterial leakage into and from prefabricated screw-retained crowns on implants in vitro. Int J Oral Maxillofac Implants. 1999; 14:654-660.
5. Persson L G, Lekholm U, Leonhardt A, Dahlén G, Lindhe J. Bacterial colonization on internal surfaces of Branemark system implant components. Clin Oral Impl Res.1996: 7:90-95.
6. Do Nascimento C, Barboza RE, Issa JPM, Watanabe E, Ito IY, Albuquerque Jr RF. Bacterial leakage along the implant-abutment interface of premachined or cast components. Int J Oral Maxillofac Implants. 2008; 37:177-180
7. Quirynen M, Bollen CML, Eyssen H, van Steenberghe D. Microbial penetration along the implant components of the Branemark system, an in vitro study. Clin Oral Implant Res. 1994; 5: 239-244.
8. Assenza B, Tripoldi D, Scarano A, Perrotti V, Piatelli A, Iezzi G, D´Ercole S. Bacterial Leakage in Implants with Different ImplantAbutment Connections: An In Vitro Study. J.Periodontol. 2012;83:491-497.
9. Koutouzis T, Wallet S, Calderon N, Lundgren T. Bacterial Colonization of the Implant-Abutment Interface Using an In Vitro Dynamic Loading Model. J.Periodontol. 2011;82:613-618.
10. Ricolmini A, Fernández FS, Straioto FG, Silva WJ, Del Bel Cury AA, Preload loss and bacterial penetration on different ImplantAbutment connection systems. Braz.Dent J. 2010;21(2)123-129.
11. Steinebrunner L, Wolfart S, Bobmann K, Kern M, In Vitro Evaluation of bacterial leakage along the Implant-Abutment interface of different Implant systems. Int J Oral Maxillofac Implants 2005;20:875-881.
12. Cortizo MC, Oberti TG, Cortizo MS, Cortizo AM, Fernandez Lorenzo de Mele M. Chlorhexidine delivery system from titanium/polybenzyl acrylate coating: Evaluation of cytotoxicity and early bacterial adhesion. J Dent. 2012; 40: 329-337
13. Quirynen M, De Soete M, van Steenberghe D. Infectious risks for oral implants: a review of the literature. Clin Oral Impl. Res. 2002; 13: 1-19.
14. Groenendijk E, Dominicus J, Moorer W, Van Waas M. Microbiological and clinical effects of clorhexidine enclosed in fxtures of 3i-Titamed implants. Clin Oral Impl Res. 2004; 15: 175-179
15. Santos Barboza RE, do Nascimento C, Mardegan JP, Watanabe E, Yoko I, de Albuquerque RF, Bacterial cultura and DNA checkerboard for the detection of internal contamination in dental implants. J Prosthodon. 2009; 18:376-381.
16. Koyanagi T, Sakamoto M, Takeuchi Y. Analysis of microbiota associated with peri-implantitis using 16 S rRNA gene clone library. JOral Microbiol. 2010; 24(2). Doi: 10.3402/jom.v2i0.5104.
17. Bürgers R, Gerlach T, Hahnel S, Schawartz F, Handel G. In vivo and in vitro bioflm formation on two different titanium implant surfaces. Clin Oral Implants Res. 2010; 21(2):156-64.Epub 2009,Nov,13.
18. Duarte PM, Reis AF, de Freitas PM, OtaTsukuki C. Bacterial adhesion on smooth and rough titanium surfaces after treatment with different instruments. J.Periodontol. 2009; 80(11):1824-32.
19. Lee A, Wang HL. Bioflm related to dental implants. Implant Dent. 2010; 19(5):387-93.
20. Pye AD, Lockhart DE, Dawson MP, Murray CA, Smith AJ. A review of dental implants and infection. J Hosp Infec. 2009; 72(2):104-10.Epub 2009,Mar 28.
21. Kern M, Harder S, Antimicrobial flling of implant cavities. J Prosthet Dent. 2010;103: 321-322.
22. Syed SA, Loesche WJ. Survival of human dental plaque flora in various transport media. Applied Microbiolology 1972; 24(4): 638-644
23. Gajardo M, Silva N, Gómez L, León R, Parra B, Contreras A, Gamonal J. Prevalence of Periodontopathic: Actinobacillus actinomycetemcomitans Bacteria in Aggressive Periodontitis Patients in a Chilean Population. J Periodontol. 2005; 76: 289-294.
24. Herrera D, Contreras A, Gamonal J, Oteo A, Jaramillo A, Silva N, Sanz M, Botero J, León R. Subgingival microbial profles in chronic periodontitis patients from Chile, Colombia and Spain. J Clin Periodontol. 2008;35: 106-113
2. Quirynen M, van Steenberghe D. Bacterial colonization of the internal part of two-stage implants. An in vivo study. Clin Oral Implant Res. 1993; 4: 158-161.
3. Broggini N, McManus LM, Herman JS, Medina RU, Oates TW, Schenk RK, Buser D, Mellonig JT, Cochran DL. Persistent Acute Inflammation at the Implant-Abutment Interface. J Dent Res. 2003; 82(3): 232-237.
4. Besimo C, Guindy J, Lewetag D, Meyer J. Prevention of bacterial leakage into and from prefabricated screw-retained crowns on implants in vitro. Int J Oral Maxillofac Implants. 1999; 14:654-660.
5. Persson L G, Lekholm U, Leonhardt A, Dahlén G, Lindhe J. Bacterial colonization on internal surfaces of Branemark system implant components. Clin Oral Impl Res.1996: 7:90-95.
6. Do Nascimento C, Barboza RE, Issa JPM, Watanabe E, Ito IY, Albuquerque Jr RF. Bacterial leakage along the implant-abutment interface of premachined or cast components. Int J Oral Maxillofac Implants. 2008; 37:177-180
7. Quirynen M, Bollen CML, Eyssen H, van Steenberghe D. Microbial penetration along the implant components of the Branemark system, an in vitro study. Clin Oral Implant Res. 1994; 5: 239-244.
8. Assenza B, Tripoldi D, Scarano A, Perrotti V, Piatelli A, Iezzi G, D´Ercole S. Bacterial Leakage in Implants with Different ImplantAbutment Connections: An In Vitro Study. J.Periodontol. 2012;83:491-497.
9. Koutouzis T, Wallet S, Calderon N, Lundgren T. Bacterial Colonization of the Implant-Abutment Interface Using an In Vitro Dynamic Loading Model. J.Periodontol. 2011;82:613-618.
10. Ricolmini A, Fernández FS, Straioto FG, Silva WJ, Del Bel Cury AA, Preload loss and bacterial penetration on different ImplantAbutment connection systems. Braz.Dent J. 2010;21(2)123-129.
11. Steinebrunner L, Wolfart S, Bobmann K, Kern M, In Vitro Evaluation of bacterial leakage along the Implant-Abutment interface of different Implant systems. Int J Oral Maxillofac Implants 2005;20:875-881.
12. Cortizo MC, Oberti TG, Cortizo MS, Cortizo AM, Fernandez Lorenzo de Mele M. Chlorhexidine delivery system from titanium/polybenzyl acrylate coating: Evaluation of cytotoxicity and early bacterial adhesion. J Dent. 2012; 40: 329-337
13. Quirynen M, De Soete M, van Steenberghe D. Infectious risks for oral implants: a review of the literature. Clin Oral Impl. Res. 2002; 13: 1-19.
14. Groenendijk E, Dominicus J, Moorer W, Van Waas M. Microbiological and clinical effects of clorhexidine enclosed in fxtures of 3i-Titamed implants. Clin Oral Impl Res. 2004; 15: 175-179
15. Santos Barboza RE, do Nascimento C, Mardegan JP, Watanabe E, Yoko I, de Albuquerque RF, Bacterial cultura and DNA checkerboard for the detection of internal contamination in dental implants. J Prosthodon. 2009; 18:376-381.
16. Koyanagi T, Sakamoto M, Takeuchi Y. Analysis of microbiota associated with peri-implantitis using 16 S rRNA gene clone library. JOral Microbiol. 2010; 24(2). Doi: 10.3402/jom.v2i0.5104.
17. Bürgers R, Gerlach T, Hahnel S, Schawartz F, Handel G. In vivo and in vitro bioflm formation on two different titanium implant surfaces. Clin Oral Implants Res. 2010; 21(2):156-64.Epub 2009,Nov,13.
18. Duarte PM, Reis AF, de Freitas PM, OtaTsukuki C. Bacterial adhesion on smooth and rough titanium surfaces after treatment with different instruments. J.Periodontol. 2009; 80(11):1824-32.
19. Lee A, Wang HL. Bioflm related to dental implants. Implant Dent. 2010; 19(5):387-93.
20. Pye AD, Lockhart DE, Dawson MP, Murray CA, Smith AJ. A review of dental implants and infection. J Hosp Infec. 2009; 72(2):104-10.Epub 2009,Mar 28.
21. Kern M, Harder S, Antimicrobial flling of implant cavities. J Prosthet Dent. 2010;103: 321-322.
22. Syed SA, Loesche WJ. Survival of human dental plaque flora in various transport media. Applied Microbiolology 1972; 24(4): 638-644
23. Gajardo M, Silva N, Gómez L, León R, Parra B, Contreras A, Gamonal J. Prevalence of Periodontopathic: Actinobacillus actinomycetemcomitans Bacteria in Aggressive Periodontitis Patients in a Chilean Population. J Periodontol. 2005; 76: 289-294.
24. Herrera D, Contreras A, Gamonal J, Oteo A, Jaramillo A, Silva N, Sanz M, Botero J, León R. Subgingival microbial profles in chronic periodontitis patients from Chile, Colombia and Spain. J Clin Periodontol. 2008;35: 106-113