Evaluation of the protective effect of mate consumption on genetic damage and oxidative stress in oral mucosa of smokers and non-smokers

Abstract

Objective: To evaluate the protective effect of yerba mate consumption on genetic damage and oxidative stress in oral mucosa of smokers and non-smokers. Methods: Four experimental groups will be formed (n=460) according to smoking habits and mate consumption. Bucal mucosa samples will be collected in a longitudinal follow-up, analyzing micronuclei, nuclear aberrations, and apoptosis. Immunostaining with CELLROX will be applied to quantify oxidative stress. Additionally, saliva samples will be collected for differential analysis of microbial groups present in the oral cavity. Gene expression analysis will be conducted to assess the involvement of specific components of yerba mate infusion in DNA repair, inflammation, and cell cycle control, as well as the prevalence of pathogenic microorganisms in the oral cavity. The results will be correlated with a standardized survey on dietary habits among mate consumers and smokers. Expected Results: A significant reduction in cellular damage is expected in mate consumers, providing evidence of its protective effect and potential use as a molecular modulator.